NRL "Cell cultures, rickettsia and oncogenic viruses"

NRL "Cell cultures, rickettsia and oncogenic viruses"

Head of Laboratory - Assoc. Prof. Petya Genova-Kalou, PhD, virologist



 

Laboratory staff:

Ekaterina Raycheva Mihailova - senior medical laboratory assistant

Katya Atanasova Georgieva-Dimitrova - laboratory assistant

Dr. Snezhana Dikova – trainee doctor

Dragana Angelov - trainee biologist

Nina Kamenova - laboratory worker

 

20161213 122501

Contact:
National Center for Infectious and Parasitic Diseases (NCIPD)
Department of Virology, NRL "Cell cultures, rickettsia and oncogenic viruses"
Bul. "Gen. Stoletov”44A, Sofia 1233
Phone number 02/9313818; 02/9313817

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Working time: 07:30 am to 14:30 pm

Laboratory "Cell cultures, rickettsia and oncogenic viruses" was established in 2019 by the merger of NRL "Herpes and oncogenic viruses" and NRL "Rickettsiae and cell cultures". The laboratory carries out diagnostic, research, educational and organizational-methodological activities in the field of herpes and papilloma virus infections, rickettsia and chlamydia infections.

Diagnostic activity
In the NRL "Cell cultures, rickettsia and oncogenic viruses" diagnostic tests are performed dignostic tests for:
- Herpes simplex virus type 1 (HSV 1)
- Herpes simplex virus type 2 (HSV 2)
- Varicella/Herpes zoster virus (VZV / HZV)
- Cytomegalovirus (CMV)
- Epstein-Barr virus (EBV)
- Human herpes virus type 6 (HHV-6)
- Coxiella burnetii
- Rickettsia conorii
- Chlamydophila pneumoniae
- Chlamydia psittaci
- Chlamydia trachomatis
- Mycoplasma pneumoniae

Diagnosis is performed by classical and contemporary molecular-biology techniques (ELISA, PCR and IFA).


Table 1. Proposed tests depending on the infection

Virus Clinic Appropriate study/ clinical materials Applied tests
Herpes simplex virus (HSV) type 1 and type 2 Labial and genital herpes, gingivostomatitis; ocular pathology, neuropathology - encephalitis, meningitis, myelitis, etc., pneumonia, hepatitis, esophagitis, etc., neonatal herpes Serum, whole blood, materials from skin and genital lesions, eye secretions, urethral and vaginal secretions, cerebrospinal fluid, biopsy tissue, etc.

ELISA – detection of IgG and IgM antibodies to HSV-1 and HSV-2 infections

PCR - detection of HSV-1 / HSV-2 DNA
Varicella / Herpes zoster virus (VZV/HZV) Varicella, herpes zoster, ocular pathology, neuropathology, congenital varicella syndrome, perinatal varicella Serum, skin rash, eye secretions, cerebrospinal fluid, amniotic fluid, biopsy tissue, etc.

ELISA – detection of IgG and IgM antibodies to VZV/ HZV

PCR - detection of VZV / HZV DNA
Cytomegalovirus (CMV) Mononucleosis syndrome, neuropathology, ocular pathology, hepatitis, esophageal and gastrointestinal ulcerations, congenital CMV infection Serum, whole blood, cerebrospinal fluid, eye secretions, urine, amniotic fluid, biopsy tissue, etc.

ELISA – detection of IgG and IgM antibodies to CMV

PCR - detection of CMV DNA
Epstein-Barr virus (EBV) Infectious mononucleosis, neuropathology, hepatitis, nasopharyngeal carcinoma, lymphoproliferative diseases Serum, whole blood, cerebrospinal fluid, biopsy tissue, etc.

ELISA - detection of antibodies to EBV: VCA IgG, VCA IgM, EA IgG, EA IgM and EBNA

PCR - detection of EBV DNA
Human herpes virus type 6 (HHV-6) Exanthema subitum (roseola infantum), neuropathology, lymphoproliferative diseases, multiple sclerosis, chronic fatigue syndrome Serum, whole blood, cerebrospinal fluid, biopsy tissue, etc. PCR - detection of HHV-6 DNA

 Coxiella burnetii

 Q fever

Acute form of Q fever

Chronic form of Q fever
Serum, whole blood, plasma, biopsy tissue, bronchoalveolar lavage, urine, removed heart valves and prostheses, pleural puncture, ticks in all stages of development

ELISA - detection of IgG and IgM phase II and phase I antibodies to Coxiella burnetii; IFA - determination of IgG and IgM phase II and phase I antibodies to Coxiella burnetii

PCR - detection of Coxiella burnetii DNA
 Rickettsia conorii Marseille fever Serum, whole blood, plasma, biopsy tissue, skin rash, ticks in all stages of development  ELISA – detection of IgG and IgM antibodies to Rickettsia conorii
 Mycoplasma pneumoniae Atypical pneumonia, neuropathology - encephalitis, meningitis, cardiovascular complications - pericarditis, myocarditis, skin complications - erythematous, maculopopular and bullous rashes, exanthemas, arthropathies Serum, whole blood, plasma, bronchoalveolar lavage, throat secretions, sputum, biopsy tissue, etc.

ELISA – detection of IgG and IgM antibodies to Mycoplasma pneumoniae

PCR - detection of Mycoplasma pneumoniae DNA
 Chlamydophila pneumoniae Atypical pneumonia, neuropathology - encephalitis, meningitis, cardiovascular complications - pericarditis, myocarditis, skin complications - erythematous Serum, whole blood, plasma, biopsy tissue, bronchoalveolar lavage, nasopharyngeal secretions, sputum, etc.

ELISA – detection of IgG and IgM antibodies to Chlamydophila pneumonia

PCR - detection of Chlamydophila pneumoniae DNA

 

NRL "Cell cultures, rickettsiae and oncogenic viruses" is accredited according to BDS / ISO 17025: 2006 as Sector "Rickettsia and Chlamydia" and Sector "Herpes viruses" of LIC of NCIPD. Every year the quality of the diagnostic work in the laboratory is controlled and certified by the Institute for Standardization and Documentation of Medical Laboratories INSTAND, Düsseldorf, Germany. The laboratory has a collection of over 50 different types of human and animal cell lines, necessary for the routine diagnosis of NRL "Enteroviruses" and NRL "Influenza and ARD", as well as for the implementation of research activities.

Organizational and methodological activity

In its capacity of NRL "Cell cultures, rickettsia and oncogenic viruses" organizes and conducts twice a year quality control of diagnostic work of virological laboratories in the country. The laboratory maintains contact and cooperates with the microbiological laboratories of RHI, MHAT, DCC and private microbiological and virological laboratories, the microbiological laboratories of infectious diseases hospitals in the country, the departments of microbiology and virology of medical universities in Sofia, Varna, Plovdiv, Pleven and Stara Zagora. and with the Laboratory of Rickettsia and Chlamydia Infections at the Bulgarian Food Safety Agency (BFSA), National Diagnostic Research Veterinary Medical Institute, Sofia.

Learning activity

The laboratory conducts specialized courses under the auspices of SDO for the acquisition of the medical specialty "Virology" of doctors and biologists. It is organized annually outside the optional training of students, postgraduates and doctoral students, as well as undergraduate internship and preparation of diploma theses of masters in various specialties. In the laboratory three diploma theses were successfully made and defended by students of the Faculty of Biology of Sofia University “St. Kliment Ohridski ”and two students from UCTM - Sofia for obtaining bachelor’s and master’s degrees. There is currently one full-time doctoral student in virology in the laboratory.

Scientific activity

The laboratory works and annually presents research projects for funding from the Research Fund of the Ministry of Education and Science. Participates in the implementation of national and international projects. The laboratory specializes in cytotoxicity testing, as well as in performing anti-viral testing of newly synthesized substances and natural products in vitro. He regularly publishes the results of his work in specialized international and Bulgarian scientific journals and reports at national and international scientific forums.

Participation in projects:

1.Research project on the topic: "Study of the chemical composition, antiviral and antibacterial activity of extracts from the plant Graptopetalum paraguayense E. Walter (Crassulaceae)", “Scientific Research Fund” Competition for financing scientific research - 2017 ”, Contract № DN19 / 16, 20.12.2017 Head of the partner organization: Assoc. Prof. Petia Genova-Kalou, PhD. Term of implementation: 2018 - 2020.

2. Research project on the topic: "Molecular genetic identification and creation of an archival genomic bank of circulating human and animal C. burnetii genotypes and determining their role as particularly dangerous infectious agents causing epizootic and epidemiological outbreaks in the Republic of Bulgaria”, "Scientific Research Fund" Competition for research funding - 2019. " Supervisor: Assoc. Prof. Petia Genova-Kalou, PhD. Term of implementation: 2019 - 2022.


Important publications:

  1. Genova-Kalou P., Vladimirova N., Stoitsova S., Krumova S., Kurchatova A., Kantardjiev T. (2019). Q fever in Bulgaria: laboratory and epidemiological findings on human cases and outbreaks, 2011 to 2017. Euro Surveill., 24(37): pii = 1900119. https://doi.org/10.2807/1560-7917 (IF = 7.4)
  2. Zaharieva M., Genova-Kalou P., Dincheva I., Badjakov I., Krumova S., Enchev V., Najdenski H., Markova N. (2019). Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study. Biotechnology and Biotechnological Equipment, 33(1): 1251 – 1259. doi: 10.1080/13102818.2019.1656108 (IF = 1.097)
  3. Krumova St., Pavlova A., Yotovska K., Genova-Kalou P. (2019). Combined laboratory approach to detection of Parvovirus B19 and Coxiella burnetii in patients with fever of unknown origin. Clinical Laboratory, 65: 69 – 76. (IF = 0.955)
  4. Ivanova Stefka, Petia Genova-Kalou, Daniela Pencheva, Zafira Mihneva. A combined serological and molecular approach to the laboratory testing of patients with reactive arthritis - detection of parvovirus B19 and Chlamydia trachomatis. Comp. Ren.de l’Acad. Bulg. Des. Sci., 2016, 69 (2), 197 - 202. (IF = 0.284)
  5. Stefka Kr. Ivanova, Petia D. Genova-Kalou, Silvia Em. Voleva, Svetla G. Angelova, Borislav M. Marinov, Viktor Em. Manolov, Stoian Shishkov. Infectious Agents and Miscarriage in Bulgaria. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 2016, 25(1), 1 - 10. (IF = 0.296)
  6. Voleva S., Ivanova S., Marinov B., Genova-Kalou, V. Manolov, V. Vasilev. New approach in the diagnostic algorithm of infectious agents (parvovirus B19 and Chlamydia trachomatis) involved in the development of pathological pregnancy. Obstetrics and Gynecology, 2016, 55(3), 3 – 10.
  7. Adelina P. Pavlova, Stefka Kr. Ivanova, Petia D. Genova-Kalou. The role of some viral and rickettsial agents in the development of unclear febrile illness accompanied by a rash. Medical Magazine, 2016, 35, 38 - 44.
  8. Pencheva, D., Genova-Kalou, P., Bryaskova, R. Comparative study of methods for inactivation of vaccines in development process of veterinary “ghost” vaccine. Comptes Rendus de L'Academie Bulgare des Sciences, 2016, 69(5), 675 - 682. (IF = 0.284)
  9. Daniela Pencheva, Tzvetan Velinov, Petia Genova-Kalou, Todor Kantardjiev. The hybrid nanomaterial PVA/AgNps, as biologically active product with reserved space in the antimicrobial therapy. Letters in Applied NanoBioScience, 2016, 5 (3): 422 – 438.
  10. Pencheva D., Velichkova E., Sandarov D., Cardoso A., Mileva M., Genova-Kalou P., Bryaskova R. Experimental Research of “Ghost” Escherichia Coli O 104. Journal of Vaccines and Vaccination, 2015, 6 (4), 4 pages.
  11. Pencheva D., Velichkova E., Sandarov D., Cardoso A., Mileva M., Genova-Kalou P., Bryaskova R. Experimental study on the components in poly-valent "ghost" Salmonella vaccine for veterinary use. Journal of Nanomaterials, 2015, Vol. 2015 (2015), Article ID 101464, 4 pages. (IF = 1.644)
  12. Radka Argirova, Raina Nenova, Dimitar Ivanov, Petia Genova-Kalou, Sevda Raleva. Experimental model to study co-infection of human immunodeficiency virus – type 1 (HIV-1IIIB) and influenza virus in cell culture. Biotechnology & Biotechnological Equipment, 2015, 6 pages. (IF = 0.300)
  13. Mileva M., Pencheva D., Bryaskova, R., Genova-Kalоu P., Kantardjiev T. Experimental treatment of recurrent otitis externa. Open Veterinary Journal, 2015, 5(1), 38 - 41.
  14. Genova-Kalou, P., J. Cherneva, M. Jordanova, A. Jordanova. The serological evaluation of the cytomegalovirus (CMV) and Chlamydia pneumoniae infections in patients with cardiovascular diseases (CVDs). J. Sc. & Techn., Medicine, 2013, 3(1), 26 - 31.
  15. Benedetti F., Berti F., Budal S., Campaner P., Dinon F., Tossi A., Genova P., Atanassov V., Hinkov A. Synthesis and biological activity of potent HIV-1 protease inhibitors based on Phe-Pro dihydroxyethylene isosters. J Med Chem, 2012, 55, 3900 – 3910. (IF = 5.589)
  16. Stankova, K. Stanoeva, A. Hinkov, I. Alexiev, P. Genova-Kalou, R. Chayrov. R. Argirova. Amino acid and peptide esters of abacavir: synthesis and activity against human immunodeficiency virus type 1 in cell culture. Med Chem Res., 2012, 21 (12), 4053 – 4059. (IF = 1.436)
  17. Genova-Kalou, P., Dundarova, D., Idakieva, K., Mohmmed, A., Dundarov, S., Argirova, R. (2008). Anti-herpes effect of the hemocyanin derived from mollusc Rapana Thomasiana. Z Naturforsch, 63с, р. 429 – 434. (IF = 0.776)
  18. Kovala-Demertzi, D., Varadinova, T., Genova, P., Souza, Demertzis, M. A. (2007). Platinum(II) and Palladium(II) Complexes of Pyridine-2-carbaldehyde Thiosemicarbazone as Alternative anti-Herpes Simplex Virus Agents. Bioinorg Chem Appl, Published online, Article ID 56165, 1 - 6. (IF = 1.265).

PRICE LIST

  Code  

Laboratory researches

Price in BGN

 103

 Venous blood collection

6,00

 311

 Qualitative determination of IgM antibodies by ELISA for HSV-1

17,00

 311

 Qualitative determination of IgG antibodies by ELISA for HSV-1

17,00

 312

 Qualitative determination of IgM antibodies by ELISA for VZV

17,00

 312

 Qualitative determination of IgG antibodies by ELISA for VZV

17,00

 313

 Qualitative determination of IgM antibodies by ELISA for EBV

17,00

 313

 Qualitative determination of IgG antibodies by ELISA for EBV

17,00

 314

 Qualitative determination of IgM antibodies by ELISA for CMV

17,00

 314

 Qualitative determination of IgG antibodies by ELISA for CMV

17,00

 314

 Determination of CMV-IgG antibodies by ELISA / avidity

17,00

 360

 Qualitative determination of IgM antibodies by ELISA for HSV-2

16,00

 360

 Qualitative determination of IgG antibodies by ELISA for HSV-2

16,00

 237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of HSV-1 DNA

41,00

 237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of HSV-2 DNA

41,00

 237

 Demonstration of DNA with polymerase chain reaction - qualitative determination of VZV

41,00

 237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of CMV

41,00

 237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of EBV DNA

41,00

 237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of HHV-6 DNA

41,00

 237

 Detection of DNA by polymerase chain reaction - detection of HPV DNA

41,00

319

 Qualitative determination of IgM antibodies by ELISA for Chlamydia trachomatis

17,00

365

 Qualitative determination of IgG antibodies by ELISA for Chlamydia trachomatis

17,00

366

 Qualitative determination of IgA antibodies by ELISA for Chlamydia trachomatis

17,00

364

 ELISA diagnosis of Q fever Phase II IgM antibodies

17,00

364

 ELISA diagnosis of Q fever phase II IgG antibodies

17,00

318

 ELISA diagnosis of Q fever Phase I IgA antibodies

17,00

318

 ELISA diagnosis of Q fever Phase I IgG antibodies

17,00

237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of C. burnetii DNA

41,00

319

 Qualitative determination of IgM antibodies by ELISA for Chlamydia pneumoniae

17,00

365

 Qualitative determination of IgG antibodies by ELISA for Chlamydia pneumoniae

17,00

366

 Qualitative determination of IgA antibodies by ELISA for Chlamydia pneumoniae

17,00

348

 ELISA diagnosis of Mycoplasma pneumoniae IgM antibodies

17,00

349

 LISA diagnosis of Mycoplasma pneumoniae IgA antibodies

17,00

350

 ELISA diagnosis of Mycoplasma pneumoniae IgG antibodies

17,00

346

 ELISA diagnosis of Marseille fever IgM antibodies

17,00

347

 ELISA diagnosis of Marseille fever IgG antibodies

17,00

237

 Demonstration of DNA by polymerase chain reaction - qualitative determination of R. conorii DNA

41,00

227

 Indirect immunofluorescence test for the detection of IgM antibodies to chlamydia

28,00

227

 Indirect immunofluorescence test for the detection of IgG antibodies to chlamydia

28,00

227

 Indirect immunofluorescence test for the detection of IgM / IgA / IgG antibodies to C. burnetii phase II

28,00

227

 Indirect immunofluorescence test for IgG antibodies to C. burnetii phase II / I

28,00

 

The site of National Centre of Infectious and Parazitic Diseases uses cookies to interact with its users.