NRL of Tuberculosis

National Reference TB Laboratory (NRL TB)

Bacijska 

 

Head of NRL TB: Senior Assistant Elizabeta Bachiyska, MD, PhD

Address: 44A, Stoletov Blvd

               1233 Sofia

               Bulgaria

Tel.:+359 2 944 64 45

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

StaffofNRLTB: Yana Todorova, biologist, Senior Assistant, PhD

                               Yuliana Atanasova, biologist

                               AnaBaykova, biologist

                               Stanislava Yordanova, biologist

                               GabrielaVasileva, lab worker

 

TBC Staff

 

History of NRL TB

NRL TB was established in 2005 as a part of the Microbiological Department, NCIPD. The laboratory is ISO 17 025 certified and also certified for its regular participation in the TB External Quality Assurance Scheme of two international organization: WHO, SRL, Antwerp, Belgium and INSTAND e.V., Dusseldorf, Germany.

 

Main activities of NRL TB

 

  • Routine diagnosis of TB: using conventional and molecular methods – from sample accepting through smear microscopy and culture to strain isolation, species identification and drug susceptibility testing. The performed microbiological analysis are: smear microscopy – Ziehl-Neelsen; culture – solid medium: Löwenstein-Jensen and liquid medium, BACTEC MGIT 960 System; DST FLD – liquid medium, BACTEC MGIT 960 System; DST SLD – liquid medium, BACTEC MGIT 960 System; LPA – Geno Type, HAIN Lifescience: MTBDRplus, MTBDRsl, CM, AS,MTBC.

 

  • Reference activity: confirmation of M/XDR-TB and NTM strains from all over the country. Twice a year NRL TB prepares panel testing for national TB network and performs EQA TB scheme for smear microscopy, culture and DST, according to Bulgarian Microbiological Standard.

 

  • Training courses: yearly 5 days training for biologists, doctors and lab technicians on microbiological diagnosis of TB and NTM.

 

  • Research: NRL TB participates in national and international scientific projects; congresses, conferences.

 

   Publication of the staff of NRL TB:

 

  1. 1.А. Йонков, А. Лачева, А. Христова, А. Торосян, А. Петкова, Б. Маринов, Б. Шентов, Б. Върбанова, В. Исаев, В. Стоянова, Г. Хаджигеоргиев, Д. Вичева, Д. Михайлова, Д. Вучев, Д. Калайков, Д. Илучев, Е. Бачийска, Е. Илиева, Е. Маркова, И.Иванова, И. Гълъбов, И. Янков, И. Чакъров, И. Галева, И. Kостадинова, И. Коева, И. Генева, К. Костов, М. Мурджева, М. Цонзарова, М. Сасова, М. Бошева, О. Бойкинова, П. Переновска, П. Чакърова, С. Филчев, Т. Дерменджиев, С. Костянев, Т. Шмилев, Х. Мумджиев, Х. Михов, Ц. Люцканова, Ц. Цолов, Клинична пулмология в детска възраст, 2013, ISBN: 978-954-9666-60-1, 171-183.
  1. П. Минчев, П. Василева, Д. Костадинов, Б. Богов, А. Алексиев, З. Спасова, М. Цонзорова, К. Праматаров, Е. Бачийска, Св. Велизарова, Централна медицинска библиотека, МУ – София, 2013, Клинична туберкулоза, Съвременна микробиологична диагностика на туберкулозното заболяване”, ISBN: 978-954-9318-29-6, 87-99.
  2. В. Миланов, Д. Стефанова, М. Замфирова, Т. Върлева, Е. Бачийска, А. Колева, А. Янева, Aнализ на факторите, свързани с изхода от лечението на случаите с мултирезистентна туберкулоза в България за периода 2009-2011г., Торакална медицинa, ISSN 1313-9827, том VI, декември 2014, брой 4, стр. 8-22.
  3. S. Yordanova, E. Bachiyska, Y. Atanasova, Y. Todorova, A. Baikova, T. Kantardjiev, Multidrug resistant tuberculosis in Bulgaria-microbiological aspects, Probl. Inf. Parasit. Dis., ISSN 0204-9155, Vol 41, Num1/2013, 5- 8.
  4. Y. Atanasova, E. Bachiyska, S. Yordanova, Y. Todorova, A. Baykova, T. Kantardjiev, Rapidly growing mycobacteria in suspected for tuberculosis pacients in Bulgaria, Probl. Inf. Parasit. Dis., ISSN 0204-9155, Vol 4, Num 2/2014, 14-16.
  5. S. Yordanova, E. Bachiyska, Y. Atanasova, Y. Todorova, A. Baikova, S. Panaiotov, T. Kantardjiev, MDR-TB with additional fluoroquinolone resistance in Bulgaria, Probl. Inf. Paras. Dis., Vol 43, Num 2/2015, 8-11.
  6. Michel Kiréopori Gomgnimbou, Iván Hernández-Neuta, Stefan Panaiotov, Elizabeta Bachiyska, Juan Carlos Palomino, Anandi Martin, Patricia del Portillo, Guislaine Refregier and Christophe Sola Tuberculosis-Spoligo-Rifampin-Isoniazid Typing: an All-in-One Assay Technique for Surveillance and Control of Multidrug-Resistant Tuberculosis on Luminex Devices, J. Clin. Microbiol, 2013, ISSN: 0095-1137, vol. 51 no. 11, 3527-3534.
  7. A Sanchini, L Fiebig, F Drobniewski, W Haas, E Richter, V Katalinic-Jankovic, E Pimkina, G Skenders, D M Cirillo, V Mathys, E Bachiyska, D Pieridou-Bagatzouni, I Zemanová, T Kummik, M Marjamäki, S Rüsch-Gerdes, D Papaventsis, P Ioannidis, N Szabó D Zallocco, L Codecasa, E Mazzola, T Mannsåker, A Santos-Silva, M Žolnir-Dovč, S Samper, M Soledad-Jimenez, K Ängeby,Y Balabanova, Laboratory diagnosis of pediatric tuberculosis in the European Union/European Economic Area: analysis of routine laboratory data, 2007 to 2011, Eurosurveillance, 2014, ISSN: 1025-496X, Vol.19, Issue 11, 1-10.
  8. J L De Beer, C Ködmön, M J van der Werf, J van Ingen, D van Soolingen, I Zemanova, D Pieridou-Bagkatzouni, A Agejeva, P Ioannidis, T Herczeg, U R. Dahle, M Poľanová, E Bachiyska, U Bidovec-Stojkovic, T Brown,D M. Cirillo, M Fauville-Dufaux, M M. Fitzgibbon, R Groenheit, S Niemann, M Obrovac, E M. Rasmussen,S Samper, W Sougakoff, P Stakenas, Molecular surveillance of multi- and extensively drug-resistant tuberculosis transmission in the European union from 2003 to 2011, Eurosurveillance, 2014, ISSN: 1025-496X, Vol. 19, Issue 11, 1-9.
  9. Sarah Sengstake, Nino Bablishvili, Anja Schuitema, Nino Bzekalava, Edgar Abadia, Jessica de Beer, Nona Tadumadze, Maka Akhalaia, Kiki Tuin, Nestani Tukvadze, Rusudan Aspindzelashvili, Elizabeta Bachiyska, Stefan Panaiotov, Christophe Sola, Dick van Soolingen, Paul Klatser, Richard Anthony and Indra Bergval Title : Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates, Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates, BMC Genomics 2014, ISSN: 1471-2164, 15 (1):572.
  10. Stefan Panaiotov, Elizabeta Bachiyska, Stanislava Yordanova, Yuliana Atanasova, Nadia Brankova, Viktoria Levterova, Sarah Sengstake, Richard M. Anthony, Indra Bergval, Christophe Sola, and Todor Kantardjiev, Beijing Lineage of MDR Mycobacterium tuberculosis in Bulgaria, 2007–2011, Emerging Infectious Diseases, ISSN: 1080-6059, Vol. 20, No. 11, 1899-1901.
  11. Vladimir Milanov, Dennis Falzon, Mariya Zamfirova, Tonka Varleva, Elizabeta Bachiyska, Antoniya Koleva, Masoud Dara, Factors associated with treatment success and death in cases with multidrug-resistant tuberculosis in Bulgaria, 2009–2010, International Journal of Mycobacteriology, 2015, ISSN: 2212-5531, 4, 131 –137.

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