NRL of Mycology

 

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Chef Laboratory

Dr Iva Ognianova Filipova

e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

tel. 359/ 2 944 69 99 / ext. 205, 318, 319 / 

Composition of the laboratory:

Bubeto 2

  • Dr Lyubomira Angelova Boyanova – This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Zoya Vasileva Ivanova,microbiologist-e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Liliana Petrova Lozanova, Medical Assistant
  • Lubka Dimitrova Kova4eva, Medical Assistant
  • Dobrinka Marinova - Medical Assistant
  • Maria Borisova – lab worker

History of the laboratory

This laboratory was found in 1988 by Prof. Dr. Todor Kantardjiev, from 1991 to 1993 it was united with the Laboratory of EIA of NCIPD. The main task of the laboratory is the organization of an interlaboratory network of microbiological laboratories in the country for the diagnosis of skin-mucous membranes and systemic mycoses with respect to public health.

• The Laboratory was designated the National Reference Laboratory of Mycology in 2001 with an order from the Ministry of Health, and from 2012 the activity is added to the supervision and reference diagnosis of sexually transmitted infections (STIs) - syphilis, urogenital chlamidosis, gonococcal, mycoplasmal and ureaplasm infections.

• Since 2002 the laboratory participates in the international quality control system and is certified by INSTAND, Düsseldorf, Germany. Since 2002, the laboratory performs external quality control for the country on laboratory diagnosis of skin-mucous mucous membranes and invasive fungal infections and from 2013 onwards for serological diagnosis of syphilis. In 2002, the laboratory is accredited under BDS / ISO 17 025 as the Mycology Division of the National Center for Infectious Diseases and is the only medical laboratory in Bulgaria with accreditation under BDS 17 025.
Diagnostic tests conducted in the laboratory

• Serological tests:
In the laboratory, serological tests for the detection of antibodies to Aspergillus, Cryptococcus and Candida by indirect immunofluorescence (IIF) in serum of patients, as well as Latex Pastorex tests for the detection of Cryptococcus and Candida antigen in serum, BAL and liquor. A method for the detection of Aspergillus galactomannan antigen and Candida mannan antigen in human serum or plasma was introduced into the laboratory by an immunoassay method, the Sandwich (ELISA) method. Patients for tropical mycoses, including endemic mycotic diseases, such as coccidioidomycosis, histoplasmosis, blastomycosis and paracoccidioidomycosis, with evidence of antibodies to radially immunodiffusion in agar, are also being studied.

• Research on dermatophytosis(Trichophyton, Microsporum, Epidermophyton)
Dermatophytes can be successfully diagnosed by direct microscopy and culturing of skin, crust, hairs and nail materials. The collection of leather, hair and nail materials can be done in sterile containers. The nail material is taken by scraping off the affected area. Collecting flakes from skin lesions is done by scraping, using a dull scalpel, in the area of the outer edge of the lesion, where the fungal agent is most likely to be still alive. Samples taken from the scalp should contain hair, plucked hair follicles and skin flakes.

Sexually Transmitted Infections Research

• Investigation for sexually transmitted chlamydial infection
Appropriate for testing is the first portion of the first morning urine, and a sample can be taken with a man's urethral swab or from the cervix (preferably in women). The study is performed by modern Molecular Diagnostic Methods (Real Time PCR) and proves the presence / absence of chlamydial DNA in the patient sample

• Study on gonorrhea
Appropriate for testing is the first portion of the first morning urine, and a sample can be taken with a man's urethral swab or from the cervix (preferably in women). The study is performed by microscopic observation of a Gram preparation, microbiological culture of special nutrition media, antibiotic susceptibility testing, and also by Molecular Diagnostic Methods (Real Time PCR), which demonstrate the presence / absence of gonococcal DNA in the patient sample.

• Sexually Transmitted Mycoplasma / Ureaplasma Infection Study
Appropriate for testing is the first portion of the first morning urine, and a sample can be taken with a man's urethral swab or from the cervix (preferably in women). The study is performed using modern Molecular Diagnostic Methods (Real Time PCR) and demonstrates the presence / absence of mycoplasma / ureaplasm DNA in the patient sample.

The Laboratory organizes annually and 2 courses under the Specialized Microbiology course "Actually Etiological Diagnosis and Etiotropic therapy of Mycosis" and "Actually Etiological Diagnostics and Etiotropic Therapy of Infections in Immunocompromised Patients".

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