Theoretical course for beginners
Sofia, NCIPD, 13 - 15.04.2010
PROGRAM
30.03 (02.04)
- 09.00 – 10.00
- Opening. History and principles of flow cytometry, (Prof. Taskov).
- 10.15 – 11.30
- Basic flow cytometer configuration (fluidic system, optical system, electronics). Last generation systems for flow cytometry analysis and cell sorting, (Prof. Taskov).
- 11.45 – 12.30
- Fluorescence and fluorochromes in flow cytometry. Principles of multi-color analysis. (A. Mihova)
- 13.30 – 14.30
- Possibilities of flow cytometry analysis (cells – subcellular fractions – soluble analytes), (Prof. Taskov, assoc.prof. M. Nikolova).
31.03 (03.04)
- 09.00 – 09.30
- Monoclonal antibodies. Immunophenotyping. Analysis of surface and intracellular markers, (Assoc.prof. M. Nikolova).
- 10.45 – 12.00
- Flow cytometry analysis of cellular functions – I (activation, proliferation, cytokine expression), (Assoc.prof. M. Nikolova, M. Muhtarova).
- 13.00 – 14.30
- Flow cytometry analysis of cellular functions – II (migration, phagocytosis, oxidative burst, allergen-specific degranulation) (Assoc.prof. M. Nikolova, D.Stankulova).
- 15.00 – 16.30
- Apoptosis, (A. Mihova, M.Muhtarova).
01.04 (04.04)
- 09.00 – 10.00
- Analysis of DNA and cell cycle, (A. Mihova).
- 10.15 – 11.15
- Clinical applications of flow cytometry (Assoc. prof. M.Nikolova)
- 11.30 - 12.30
- Design of flow cytometry experiments: planning, performing, data analysis and publication, (Prof. Taskov).
- 13.30 – 14.30
- Discussion and closure, (Prof. Taskov).
Registration:
Registration fee: 45 lv. The number of participants is limited!
For additional information and registration:
- Dr. Maria Nikolova, Antoaneta Mihova
- tel.
- 359 2 943 56 36
- This email address is being protected from spambots. You need JavaScript enabled to view it.
COMING SOON:
Practical Course: Flow Cytometry DNA Analysis, May and October 2010
Practical Course: Flow Cytometry Analysis of Apoptosis, June 2010