INTRODUCTION TO FLOW CYTOMETRY

Theoretical course for beginners

Sofia, NCIPD, 13 - 15.04.2010

PROGRAM

30.03 (02.04)
09.00 – 10.00
Opening. History and principles of flow cytometry, (Prof. Taskov).
10.15 – 11.30
Basic flow cytometer configuration (fluidic system, optical system, electronics). Last generation systems for flow cytometry analysis and cell sorting, (Prof. Taskov).
11.45 – 12.30
Fluorescence and fluorochromes in flow cytometry. Principles of multi-color analysis. (A. Mihova)
13.30 – 14.30
Possibilities of flow cytometry analysis (cells – subcellular fractions – soluble analytes), (Prof. Taskov, assoc.prof. M. Nikolova).
31.03 (03.04)
09.00 – 09.30
Monoclonal antibodies. Immunophenotyping. Analysis of surface and intracellular markers, (Assoc.prof. M. Nikolova).
10.45 – 12.00
Flow cytometry analysis of cellular functions – I (activation, proliferation, cytokine expression), (Assoc.prof. M. Nikolova, M. Muhtarova).
13.00 – 14.30
Flow cytometry analysis of cellular functions – II (migration, phagocytosis, oxidative burst, allergen-specific degranulation) (Assoc.prof. M. Nikolova, D.Stankulova).
15.00 – 16.30
Apoptosis, (A. Mihova, M.Muhtarova).
01.04 (04.04)
09.00 – 10.00
Analysis of DNA and cell cycle, (A. Mihova).
10.15 – 11.15
Clinical applications of flow cytometry (Assoc. prof. M.Nikolova)
11.30 - 12.30
Design of flow cytometry experiments: planning, performing, data analysis and publication, (Prof. Taskov).
13.30 – 14.30
Discussion and closure, (Prof. Taskov).
Registration:

Registration fee: 45 lv. The number of participants is limited!

For additional information and registration:

 
Dr. Maria Nikolova, Antoaneta Mihova
tel.
359 2 943 56 36
e-mail
This email address is being protected from spambots. You need JavaScript enabled to view it.

COMING SOON:

Practical Course: Flow Cytometry DNA Analysis, May and October 2010

Practical Course: Flow Cytometry Analysis of Apoptosis, June 2010

The site of National Centre of Infectious and Parazitic Diseases uses cookies to interact with its users.