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NRL of Enteric infections, pathogenic cocci and diphtheria

NRL of Enteric infections, pathogenic cocci and diphtheria

Head: Dr. Antoaneta Decheva, Ph.D.

tel. +359 2 9446 999 / 265;

е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Composition of the laboratory:

  • Chief Assist. Maria Radoslavova Pavlova - Biologist, Doctor of Microbiology
    e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
  • tel. +359 2 9446 999 / 268

IMG 0884 2

 

  • Lilia Todorova – lab worker

IMG 0890 1

  • Daniela Spasova - lab worker

 

History of the laboratory

In 1950 a laboratory for Shigella was funded in NCIPD, headed by Dr Trifonova. In 1961 a laboratory for E. coli was funded, headed by Dr Atanasova, also a laboratory for Salmonella, headed by Dr Koen. Their adherents were Dr Bratoeva, Dr Mladenova, Dr Mollov. The National Reference Laboratory for Enteric Infections was funded in 1993 and was headed by Dr Petar Petrov. In 2019 this laboratory was associated with the NRL for Pathogenic cocci and diphtheria.

The National Reference Laboratory of enteric infections, pathogenic cocci and diphtheria is accredited by BAS according to the standard BDS EN ISO / IEC 17025: 2006 of 31.10.2009.

Activities of the laboratory

Diagnostic work

Processing throat swabs, nasal swabs and feces for identification of pathogenic cocci, corynebacteria and enteric bacteria.

The laboratory performs serologic investigation of serum samples for typhoid fever.

Reference work

Confirmative identification of isolates which are sent to the laboratory from other microbiological labs: pathogenic cocci, corynebacteria and enteric bacteria.

Education

Each year the laboratory performs courses on:

Enteric infections;

Streptococcal infections and diphtheria;

Staphylococcal infections.

Science

The laboratory participates in national and international scientific projects.

  1. FWD AMR – RefLabCap for AMR Testing in Salmonella and Campylobacter, funded by The Consumers, Health, Agriculture and Food Executive Agency, Directorate-General for Health and Food Safety, ECDC, Statens Serum Institute and the Technical University of Denmark.
  2. GRAM (Global Research on Antimicrobial Resistance). University of Oxford and Institute for Health Metrics and Evaluation (IHME), Washington.

Publications for 2018-2022

  1. Peterson, M.E.a, Li, Y.a, Bita, A.b, Moureau, A.c, Nair, H.a,d, Kyaw, M.H.e, Abad, R.f, Bailey, F.g, Garcia, I.F.h, Decheva, A.i, Krizova, P.j, Melillo, T.k, Skoczynska, A.l, Vladimirova, N.i Meningococcal serogroups and surveillance: A systematic review and survey.  Journal of Global Health Open AccessVolume 9, Issue 1, 2019, Article number 010409. IF 4.195 Q1   
  2. Florens Lohrmann, Markus Hufnagel, Mirjam Kunze, Baharak Afshar, Roberta Creti, Antoaneta Detcheva, Jana Kozakova, Javier Rodriguez-Granger, Uffe B. Skov Sørensen, Immaculada Margarit, Domenico Maione, Daniela Rinaudo, Graziella Orefici, John Telford, Manuel de la Rosa Fraile, Mogens Kilian, Androulla Efstratiou, Reinhard Berner & Pierrette Melin for the DEVANI Study Group. Neonatal invasive disease caused by Streptococcus agalactiae in Europe: the DEVANI multi-center study. Infection, December 2022. Infection https://doi.org/10.1007/s15010-022-01965-x. IF 5.284 Q1
  3. Study on patients with Clostridioides difficile infection during the COVID-19 pandemic in BulgariaValeri. Veleva, Maria Pavlova, Ekaterina Alexandrova, Мetodi Popov, Ivan  Lutakov, Tatiana  Tcherveniakova,  Andreana  Angelova, Eli  Hristozova,  Yordan  Kalchev and  Ivan  Ivanov. Biotechnology & Biotechnological equipment 2023, Vol.  37, no.  1, 188–193 – Q3
  4. CAMPYLOBACTER INFECTION IN CHILDREN AND ADULTS IN BULGARIA: COMPARATIVE CHARACTERISTICS AND ANTIMICROBIAL RESISTANCE. Velev, V., Pavlova, M., Alexandrova, E., Ivanov, I., Popov, M. Biotechnology and Biotechnological Equipment, 2022, 36(1), pp. 292–298 – Q3; IF= 1.762
  5. RECURRENT MENINGITIS CAUSED BY SALMONELLA ENTERICA PARATYPHI B VAR. JAVA IN 3-MONTH-OLD INFANT. M. Pavlova, G. T. Lazarova, E. Aleksandrova, V. Velev, S. Isencik, P. Georgieva, M. A. Mitev. Paediatrics and international child health. 2022. DOI: 10.1080/20469047.2023.2168428 – Q3; IF= 2.73
  6.  PREVALENCE OF DIARRHEAGENIC E. COLI AMONG HOSPITALIZED CHILDREN IN A CLINICAL CENTRE.  V. Velev, M. Pavlova, E. Alexandrova, M. Popov, I. R. Ivanov. Acta Medica. Vol. XLVIII, 2021, №4, 5-8. DOI: 10.2478/AMB-2021-004 - Q4
  7. A STUDY OF PARATYPHOID FEVER IN BULGARIAN CHILDREN Pavlova, M., Alexandrova, E., Kalchev, Y., ...Murdjeva, M., Kantardjiev, T. Acta Medica Bulgarica, 2021, 48(1), pp. 59–62 - Q4
  8. ULTRASOUND CHANGES AS A MARKER FOR EARLY DIAGNOSIS OF HEPATITIS A IN CHILDHOOD.Velev, V., Popov, M., Velcheva, D., Pavlova, M. General Medicine, 2021, 23(6), pp. 50–53 - Q4
  9. VIRAL GASTROENTERITIS IN CHILDREN – CLINICAL AND MOLECULAR-EPIDEMIOLOGICAL FEATURES Velev, V., Popov, M., Stoyanova, A., ...Tcherveniakova, T., Nikolaeva-Glomb, L. General Medicine, 2021, 23(4), pp. 32–37 - Q4
  10. A CASE OF FASCIOLOSIS IN A 11-YEAR-OLD SYRIAN IMMIGRANT BOY Velev, V., Vutova, K., Pavlova, M., Mangarov, I. Journal of Tropical Pediatrics, 2020, 66(2), pp. 226–227 – Q2; IF = 1.794
  11.  SHIGELLOSIS IN CHILDREN-CLINICAL COURSE AND ANTIMICROBIAL SENSITIVITY Pavlova, M., Popov, M., Alexandrova, E., Velev, V. General Medicine, 2020, 22(6), pp. 33–36 – Q4
  12. CAMPYLOBACTER INFECTIONS AMONG BULGARIAN CHILDREN: MOLECULAR CHARACTERIZATION AND ANTIMICROBIAL SUSCEPTIBILITY Pavlova, M., Alexandrova, E., Donkov, G., ...Kantardjiev, T., Velev, V. Biotechnology and Biotechnological Equipment, 2020, 34(1), pp. 1038–1042 – Q3 IF= 1.632
  13.  COVID-19 AND GASTROINTESTINAL INJURY: A BRIEF SYSTEMATIC REVIEW AND DATA FROM BULGARIA. Velev, V., Popov, M., Velikov, P., ...Tcherveniakova, T., Pavlova, M. Infezioni in Medicina, 2020, 28, pp. 37–41 – Q3
  14. TONGUE INFECTION CAUSED BY DIROFILARIA REPENS Velev, V., Popov, M., Pavlova, M., Karageorgiev, M., Mangarov, A. QJM, 2019, 112(8), pp. 619–620 – Q2;
  15.  ADVANTAGES OF EVA GREEN REAL-TIME MPCR WITH CULTURE AND IMMUNOCHROMATO-GRAPHIC METHODS FOR DIFFERENTIATING C. JEJUNI / COLI DIRECTLY FROM FECES Pavlova, M., Velev, V., Dobreva, E., ...Tomova, I., Kantardjiev, T. Acta Medica Mediterranea, 2018, 34(4), pp. 1027–1030 – Q3; IF= 0.147
  16.  INVESTIGATION OF SALMONELLA ENTERITIDIS OUTBREAK IN FOUR KINDERGARTENS Pavlova, M., Velev, V., Karageorgiev, M., ...Mangarov, A., Kantardjiev, T. Infezioni in Medicina, 2018, 26(4), pp. 316–320 -Q2
  17.  DIAGNOSTICS AND THERAPEUTIC BEHAVIOUR IN PATIENTS WITH CAMPYLOBACTERIOSIS Velev, V., Pavlova, M., Mangarov, A., ...Tomov, T., Vutova, K. Comptes Rendus de L'Academie Bulgare des Sciences, 2018, 71(3), pp. 417–423 – Q2; IF= 0.326

International quality assurance

INSTAND, Düsseldorf, Germany

WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens and Community Reference Laboratory – Antimicrobial Resistance, CRL-AR, Copenhagen, Denmark

External quality assay for Bulgaria

The laboratory organizes and performs twice per year quality assurance for the microbiological laboratories in Bulgaria:

 

 

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